Stevan N Djakovic

2 Questions 5 Answers 0 Followers

Questions related from Stevan N Djakovic

For an ex vivo blood experiment, how do you incubate?

I will be treating heparinized whole blood with a small molecule for various timepoints and then extracting RNA for qPCR. Do people recommend incubations in tubes or plates/dishes? 5% CO2/37C or...

22 January 2015 5,612 3 View

What is your opinion on agitation/shaking (ie on a plate shaker)?

I am fairly new to ELISAs and had a quick question. What is your opinion on agitation/shaking (ie on a plate shaker)? Only during washes, during all incubations or never? Have you noticed a...

13 November 2012 2,834 11 View