Stephen Lippi

4 Questions 6 Answers 0 Followers

Questions related from Stephen Lippi

What is the best way to store blood from a mouse?

We are looking at collecting blood to assess resting glucose levels. However, with several animals going through terminal procedures, what is the best way to store collected blood? (Ice, dry ice);...

04 April 2019 6,803 3 View

Can you mix poly- and mono-clonal antibodies in Western Blot?

Is it ok to probe with antibodies of different clonalities at the same time? For instance, if I'm using a mouse monoclonal GSK3 antibody and also want to use a rabbit anti mouse polyclonal GAPDH...

05 May 2018 4,405 3 View

Has anyone had difficulties breeding with J20 or rTg4510 (Jax) mice?

We are currently breeding J20 males and rTg4510 females from the Jax Lab and are having breeding difficulties. We pair for 2 weeks (we also give Lovemash throughout the breeding period). Has...

09 September 2016 5,332 3 View

How much lysis buffer should I use in order to extract cytosolic proteins for hemisphere homogenates?

I'm preparing to run Western blot analyses on rat tissue and have read some estimates where ~5mg of tissue is used with 300uL of lysis buffer. However, a hemisphere of a 4-month old rat weighs...

07 July 2015 1,969 6 View