Stephen Zhang

2 Questions 1 Answers 0 Followers

Questions related from Stephen Zhang

Detached HEK293 cells not staining with trypan blue - dead or alive?

Hi, I've been performing a kill curve assay for puromycin with 293T cells. 5 days post puro addition, it appears that the vast majority of cells (90+%) had detached, forming very large 'mats' of...

24 July 2017 767 2 View

Successful PCR despite unexpectedly high annealing temperatures

Hi, I'm currently working on PCR optimisation for a ~1kb target sequence that contains a large number of inverted repeats. I am using the KAPA HiFi HotStart ReadyMix...

28 June 2017 1,600 1 View