Stephen John Mccleary

5 Questions 12 Answers 0 Followers

Questions related from Stephen John Mccleary

How can i remove cells from a 96 well culture plate to store them using RNAlater?

Basically i need to remove some monocyte macrophages from a 96 well culture plate to store them for later use, which may well be cellular gene expression analysis or more simple viral DNA...

13 February 2020 331 2 View

Are there any devices or protocols available to provide quick, efficient and reproducible production of liposomes for antigen encapsulation?

I'm looking for a simple way to encapsulate TLR agonists to use in a small pilot study.

01 February 2020 9,614 3 View

Virus inactivation problems using Qiagen AVL?

For virus extraction and inactivation validation purposes I need to find a method that will reduce cytoxicity observed on TCID50 assays while using AVL buffer. Anybody have any helpful...

30 January 2018 4,748 2 View

Can anyone suggest suitable ultracentrifugation speeds for washing PLGA nanoparticles?

I currently use an ultracentrifuge (40,000g/20mins) to wash PLGA nanoparticles following encapsulation and coating with chitosan, however the wash step in deionised water results in a considerable...

04 April 2016 1,549 4 View

Is it possible to innoculate in cell culture, with ordinarily what is a non cytopathic virus, at such a high MOI that it results in cell death?

Have been running an experiment to grow high titre virus that I have repeated twice using the same virus batch, and also repeated the experiment with a different batch of virus i.e. stocks that...

03 December 2015 4,313 8 View