Stephanie M. Miller

2 Questions 10 Answers 0 Followers

Questions related from Stephanie M. Miller

What are the optimised conditions/protocols for HIF-1a Ab for use in WB and IHC?

Hi all, I've just got in an Abcam HIF-1a Ab with the intention of running both Western Blots (frozen pig brain tissue) and Immuno (PFA fixed, paraffin embedded tissue). Just wanting to hear from...

01 January 2015 8,165 7 View

When do I add protease inhibitors to my lysate?

I'm trying to 'optimise' the way I prep brain tissue lysate for Western blots. I plan to use MMP-9 Ab- so don't want to use EDTA/EGTA in my cocktail. I'm happy to concoct my own mix (in the first...

01 January 2015 8,899 11 View