What are the conditions that you use in order to do immunostaining in differentiated 3T3-L1 cells?

Hello,I would like to do immunostainings on differentiated 3T3-L1 cells (adipocytes). We tried most of the "classic" protocols, fixation with either 4% PFA or cold methanol/acetone, and a lot of...

18 January 2017 8,674 3 View