3 Questions 1 Answers 0 Followers
Questions related from Stella Wigglesworth - Littlewood
Does anyone have experience with dephosphorylating proteins once they have been transferred onto a PVDF membrane. Do I incubate the PDVF membrane in the CIP before or after blocking? I have found...
28 November 2023 2,873 0 View
Does anyone have any experience de-phosphorylating proteins in a cell lysate before SDS PAGE and western blotting? I have a calf intestinal phosphatase, however I have heard that lamba...
25 May 2023 5,524 1 View
I am using Phos-tag SDS PAGE and subequent western blotting to determine if the protein I am researching is being phosphorylated. I am using ECL to image my blots. However, I am getting really...
31 March 2023 5,001 3 View
