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Questions related from Stella Wigglesworth - Littlewood
I did a western blot detected using the supersignal west pico plus ECL reagent. However when imaged the blot background was dark grey and the bands white? Does anyone have an explanation as to why...
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Does anyone have experience with dephosphorylating proteins once they have been transferred onto a PVDF membrane. Do I incubate the PDVF membrane in the CIP before or after blocking? I have found...
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Does anyone have any experience de-phosphorylating proteins in a cell lysate before SDS PAGE and western blotting? I have a calf intestinal phosphatase, however I have heard that lamba...
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I am using Phos-tag SDS PAGE and subequent western blotting to determine if the protein I am researching is being phosphorylated. I am using ECL to image my blots. However, I am getting really...
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