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Questions related from Stella Evans
I'd like to perform RT-PCR to distinguish between transcripts that are about 95% homologous. Does anyone have experience in determining how unique the primers must be in order to detect only the...
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I've been attempting to subclone an insert from one vector to another by PCR amplification with primers that incorporate XhoI/EcoRI sites at the ends of the insert and then digesting the insert...
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