Stefano Pessina

3 Questions 8 Answers 0 Followers

Questions related from Stefano Pessina

Where should I design the gRNA if I want to insert point mutations with CRISPR/Cas9?

I'm trying to insert few point mutations close to each other in a gene of interest and I want to use CRISPR/Cas9. Clearly, I am aiming at HDR rather than NHEJ, so I will provide a template DNA...

03 March 2016 7,844 6 View

Is anyone working on CRISPR/Cas direct delivery in plants?

Hello everyone. I am currently writing a project proposal for a postdoc at KU Leuven (Belgium). The topic is genome editing with CRISPR/Cas in banana and I would like to use direct delivery, cause...

02 February 2016 7,334 3 View

How is it possible to screen the interactions between a transmembrane protein and cytoplasmic proteins?

I have a protein of interest, that is a trans-membrane protein. I want to screen the interactions of this protein with both membrane and cytoplamic proteins. For interactions with membrane...

10 October 2014 7,287 12 View