Stefan Bidula

3 Questions 7 Answers 0 Followers

Questions related from Stefan Bidula

Differentiation of primary human monocytes to monocyte derived macrophages?

We are currently trying to differentiate primary human monocytes from donor blood into monocyte derived macrophages for phagocytosis studies. However, each attempt has failed thus far. Following...

11 April 2013 5,645 38 View

The publication process: Where to put the methods used to produce supplementary data if the method has not been used for data in the main manuscript?

I am currently in the process of additional experimentation to try and convince the reviewers (sound familiar?) to add into supplementary material. However, some of the methods I am using to...

14 December 2012 3,759 1 View

Detection of cytokines in supernatant following pathogen challenge

I have recently challenged A549 airway epithelial cells with a pathogen and collected the supernatant at set time points to be frozen and analyzed at a later date. However, we currently don't have...

14 September 2012 2,204 17 View