Stanley Zhou

5 Questions 8 Answers 0 Followers

Questions related from Stanley Zhou

Has anyone had trouble in getting bands to show up on LICOR Western Blots?

I am trying to probe for a loading control. I don't know why sometimes the bands show up and other times they don't. It's black or white whereas the actual protein I'm tagging for is consistent...

05 May 2017 7,828 3 View

SiRNA question using RNAiMAX - do you change media day after transfection?

Hello, I am still new to doing RNAi experiments, after I transfected siRNAs against my gene of interest, using siGAPDH as a positive control as well as a commercial negative control siRNA as a...

03 March 2017 454 6 View

Why is there so much background on my cloning controls (i.e no ligase, no insert and no backbone)?

Hello, I am concerned about my cloning. I am trying to clone a 1.7kb fragment into my 3kb backbone. When transformed and plated, the cloning seems to work, but the background colonies are...

11 November 2016 4,282 5 View

Recommendation for FOXA1 antibody?

Hello, I am wondering which FOXA1 antibody do you guys use to conduct your ChIP experiments? I've been using 17-10267 from Millipore. I am planning to ChIP low cell numbers (i.e

11 November 2016 8,715 4 View

Looking for advice on cloning an oligo library into a backbone?

I have my oligonucleotides synthesized on a chip. Next, I PCR amplified restriction enzyme tails into the oligo library (for blunt-end ligation later). I have been doing multiple PCR amplification...

10 October 2016 7,516 4 View