Stacy Schaefer

3 Questions 2 Answers 0 Followers

Questions related from Stacy Schaefer

Does anyone have experience with low-input kits for RNASeq library prep following unexpectedly low RNA extraction yields?

I performed an RNA extraction using the Qiagen RNEasy Micro Kit, and my total RNA yield was lower than expected given the amount of input material. I'm considering whether to move forward with...

22 April 2016 1,425 1 View

Does anyone else consistently see background bands at 60 kDa in Western blots?

I routinely see a major band at ~60 kDa in my Western blots with Ponceau staining and HRP-conjugated antibody staining. This occurs with different cell lysates (both mouse and human), and it is...

24 August 2015 3,059 3 View

Does anyone have experience with H9 Cre-LoxP cell line?

I am considering whether to purchase H9 or H9 Cre-LoxP cells. I know that H9 is a very popular cell line but haven't seen many references for the Cre-LoxP-modified version. Does anyone have...

11 August 2014 2,759 3 View