Stacey Aggarwal-Howarth

2 Questions 3 Answers 0 Followers

Questions related from Stacey Aggarwal-Howarth

Hoechst staining is dimmer only in cells over expressing my protein of interest?

In the MCF7 human breast cancer cell line, I noticed that cells which highly expressed my GFP-tagged protein of interest have much dimmer nuclear staining with Hoechst dye, to the point where you...

09 September 2016 2,487 3 View

Does anyone have experience with the dCas9-FokI for knock-in mutations? Any tips for more robust knock-in of mutations?

We've tried homologous repair with either plasmid or DNA oligo containing the mutant sequence and homology arms but have not been able to generate satisfactory levels of mutation. I also can't...

12 December 2015 6,590 0 View