Time-lapse 2-photon imaging of HEK293T cells for 1-2 hours, how do I reduce cell movement/morphology changes?

I am working on imaging a fluorescent protein expressed in HEK293-T cells with 2-photon excitation for long time durations (1-2 hours). I notice movement and changes in morphology of the cells...

28 January 2020 1,216 2 View