Srishti Chakravorty

3 Questions 6 Answers 0 Followers

Questions related from Srishti Chakravorty

Do all tumor cells express MHC-1 protein that can be detected by Flow Cytometry?

I understand that Antigen Presenting Cells (APCs) like tumor cells express MHC-1 which in most cases might be altered. Before starting my main experiment, I did a preliminary experiment to...

10 October 2019 1,181 4 View

Has anyone tried transfecting human B lymphoblastic cells (BLCLs) with MSCV plasmids?

According the Clontech, "The MSCV Retroviral Expression System (murine stem sell virus) contains vectors that are optimized for introducing and expressing target genes in pluripotent cell lines,...

08 August 2018 3,873 7 View

What if Sodium pyruvate is added in excess in DMEM cell culture media?

What happens if we accidentally add more (5 ml of 200 mM) sodium pyruvate to 500 ml DMEM cell culture media that already contains 110 mg/ml sodium pyruvate in it (from company)? How will it...

11 November 2017 3,606 3 View