Srinivas Thota

2 Questions 6 Answers 0 Followers

Questions related from Srinivas Thota

Is a lac promoter with a CRP binding site active in pseudomonas syringae DC3000?

I cloned pseudomonas genes downstream to a lac promoter in pUCP24 and pBBR1-MCS2. I am trying to do a western blot and not able to see any bands. VRF, a CRP ortholog is present in pseudomonas...

10 October 2013 5,560 0 View

Overexpression of transcription factor to perform Chip-seq?

I am trying to express a transcription factor in pseudomonas which probably might be very tightly regulated. The expression now is so low that I can barely see it on a western blot. Expression...

09 September 2013 7,592 6 View