Srinivas Allanki

7 Questions 8 Answers 0 Followers

Questions related from Srinivas Allanki

Can we culture THP1 in Minimum Essential Medium instead of the ATCC suggested RPMI?

I have seen some answers saying that we can use DMEM instead of RPMI (11.89mM Glucose). But I want to culture them in a low glucose medium (5mM Glucose) as per my experiments and we don't have low...

28 July 2015 7,377 3 View

Why are THP1 monocytes getting adhered to the dish during serum starvation?

THP1 monocytes, despite of them being a suspension culture are getting adhered (all the cells) to the dish during serum starvation (at least 45mins to 1hr in basal RPMI). They are adhering so...

20 June 2015 6,172 7 View

What are the effects of the presence of insulin in the culture medium of MCF-7?

As per ATCC guidelines, there should be a 0.01 mg/ml of insulin in the medium. Please explain if there are any noticeable changes (phenotypic and genotypic) in the cell line?

29 March 2014 2,087 0 View

Can anyone explain why neurons stop regenerating after an injury to the spinal cord in humans?

Neurons stop to regenerate after a certain age or after an injury in humans and some other higher vertebrates. But it is observed in some of the lower vertebrates. I would like to know why we were...

25 November 2013 6,725 4 View

Why do we use nested primers in TAIL PCR?

What is the difference between normal primers and nested primers and why are nested specfically used in TAIL PCR?

03 May 2013 1,551 7 View

How should I model the tea concentration as a function of time after a tea bag has been submerged?

Is it diffusion or osmosis?

16 April 2013 5,078 2 View

To what extent is using bacteriophages instead of antibiotics safe and successful?

Day by day bacteria are turning antibiotic resistant. So, the alternative that people came up with is using bacteriophages to kill specific bacteria that are causing trouble to us. How successful...

09 April 2013 7,090 4 View