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My HeLa cells are not adherent in to T-25 flask after freezing. I have been using corning tissue culture flask and my freezing medium has 10% DMSO. Does anyone have experience like this? Kindly...
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Does anyone have experience with this cell line? I bought this cell line as a frozen sample, and two months after thawing its growth is very slow. I'm using the medium composition as per ATCC .
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