Sreelakshmi Cherakara

2 Questions 2 Answers 0 Followers

Questions related from Sreelakshmi Cherakara

How detergents like Triton-X and Brij-58 helps in preventing protein aggregation ?

I was doing the purification of Human Hsp40, and many papers refer to use detergent during the purification protocol. I just wanted to know how the detergent Triton-X or Brij-58 helps in avoiding...

11 September 2020 635 6 View

Extensive cleaning of Superdex TM 200 increase 10/300 GL column?

After a series of 6-7 purifications in SuperdexTM 200 increase 10/300 column, its pressure has increased to 1.08MPa even at a very low flow rate of 0.1ml/min. And I could see collapsing of the...

01 January 1970 6,670 4 View