Sreeja V Nair

4 Questions 5 Answers 0 Followers

Questions related from Sreeja V Nair

Does N-terminal HA tag interfere in Met-1 mediated ubiquitination of HA-Ub-K0 in mammalian cells ?

I have co-transfected HEK293 cells with gene of my interest and HA-Ubiquitin wt/HA-Ubiquitin-K0 and performed a pull down experiment with FLAG. Objective of my experiment is to check the...

10 October 2017 1,611 0 View

Could anyone suggest a method to reduce background in biotin-streptavidin pull down with APEX tag constructs?

I have cloned my gene of interest in pcDNA3-CMV-APEX-NLS and this construct was used to transfect HEK293T cells.pcDNA3-CMV-APEX-NLS was used as a control during transfection.I have performed...

12 December 2015 3,464 3 View

Where can I get miR 21-/- cells?

I would like to get miR21 null cells for one of my experiment. Can anyone suggest a lab/place for the same?

02 February 2015 2,755 0 View

Can anybody help me to increase the efficiency of retroviral mediated transfection in MEF cells?

I have cotransfected pVpack GP and pVpack VSV in HEK 293 cells. Then I collected the supernatant having viral particles after 48 hrs of tranfection and used the same to infect MEF cells.

12 December 2014 9,180 6 View