Sravya Varali Kovvali

3 Questions 2 Answers 0 Followers

Questions related from Sravya Varali Kovvali

How do I label a periodate oxidized 3' end of an RNA molecule with cystamine?

I am trying to label my RNA with cystamine on it's 3' end and I have done periodate oxidation to get an aldehyde from the diols. But the cystamine labeling did not work. I followed the exact...

13 April 2018 9,383 3 View

How can I explain the loss of my protein during size exclusion chromatoography

I am trying to purify an endonuclease (N-terminal hexahistidine tagged) of about 16kDa. The first round of purification involves overnight binding with Ni-NTA beads. When the elution fractions are...

01 July 2016 506 4 View

Can expression conditions differ if the Histag of a protein has been changed from the N-Terminal to the C-Terminal?

I have been expressing an N-terminal hexahistidine tagged endonuclease in E.coli cells, Rosetta strain. Due to some issues we had with purification, we had decided to construct a C-terminal histag...

01 January 1970 1,890 3 View