Sravya Arikatla

2 Questions 4 Answers 0 Followers

Questions related from Sravya Arikatla

What is the protocol for for detecting the ROS levels in senescence dermal fibroblasts?

For detecting the ROS in dermal fibroblasts I'm using DCFH2-DA dye. By using the fluorescence microscope the ROS levels are not getting detected in the senescence dermal fibroblasts. So, is there...

01 January 1970 7,418 3 View

DCFH2-DA for the detection of ROS levels in the Primary dermal fibroblasts?

Day: 1 Seeding the cells Day: 2 Drug Treatment (incubate the cells for 24hrs) Day: 3 Wash the cells with HBSS for twice and incubate the cells with 10micromolar of DCFH2-DA(Sigma-Aldrich)...

01 January 1970 9,097 6 View