Is cloning a 9kb 3'UTR for luciferase assay necessary or cloning the regions of 3'UTR-miRNA interaction is sufficient?

Hi, the gene of my interest has a 3'UTR of nearly 9.8kb. I am planing to perform luciferase assay for studying the miRNA-target interactions. So is it okay if i could clone  those regions where...

09 February 2016 3,270 3 View