Sourajit Mukherjee

3 Questions 10 Answers 0 Followers

Questions related from Sourajit Mukherjee

Reversal potential (of a Ca2+/H+ exchanger) doesn't change with the change in concentration gradient of the ions! Am I missing anything?

I am trying to perform whole-cell patch clamp on HeLa cells over expressed with a calcium/proton exchanger. I am failing to understand why there is no shift in the reversal potential even if I...

17 May 2022 4,839 0 View

What is holding potential in electrophysiology voltage-clamp experiments? Is it related to the reversal potential of an ion channel/exchanger?

I am trying to follow this paper https://www.cell.com/biophysj/fulltext/S0006-3495(02)75543-4 for finding out the stoichiometry of one of the exchanger in question. However, even if I use a...

05 May 2022 3,412 15 View

Membrane protein expression failed in Pichia Pastoris. What can be done next?

Hello All, I am writing this post as I don't have any prior knowledge in Protein Biochemistry and Structural Biology. I am trying to express a sub-cellular membrane protein in Pichia pastoris...

19 February 2022 8,027 5 View