Is it possible to visualize green DCF fluorescence in DCFDA assay on live cells, and quantify using Hoechst dye to stain the nucleus?

I wish to perform DCFDA assay (Sigma) on primary microglia after treatment. The number of cells is so low that I cannot proceed for Flow-based analysis techniques. Therefore, I am thinking of...

08 August 2019 5,261 3 View