Sophie Briggs

3 Questions 11 Answers 0 Followers

Questions related from Sophie Briggs

What are the best concentration and timescales when pre-coating plates with Protamine Sulfate for DNA binding?

Hi all, I am using an ELISA method for detecting DNA and have established that Protamine Sulfate looks best for binding DNA to the plate. Does anyone have any suggestions for starting...

08 August 2018 7,847 1 View

Cell culture contamination with suspension cells?

I am culturing primary human brain stem cells which grow as neurospheres but I seem to constantly get what looks like bacterial contaminations and cannot seem to work out why. I have had 4 years...

05 May 2016 9,092 9 View

How can I correct unequal loading in Western Blots?

I am currently doing western blots with BTIC cells (Brain tumour initiating cells) which grow as neurospheres. I have done western blots before (with monolayer BTIC's) and had no issues...

06 June 2015 3,955 19 View