Sophie C Glen

2 Questions 2 Answers 0 Followers

Questions related from Sophie C Glen

Alternatives to Laminin 521?

I've been using poly-ornithine and laminin to coat plates for neuroepithelial stem cells from iPS. Some plates work fine but quite often I have attachment problems where all cells detach after a...

19 October 2016 4,565 3 View

When cryopreserving CF-1 MEF cells, which media should I use?

I have irradiated MEF cells which will be frozen to be used to produce feeder layers for the growth of iPS cells. I have seen two methods from various protocols; seems I should either use MEF...

05 December 2014 8,263 2 View