How to fix MCF10A cells from spontaneously transitioning to mesenchymal state?

I have ordered MCF10A cells from AddexBio 2 years ago and grew them in 1:1 DMEM/F12, 5% Horse Serum (Wisent), 10ug/mL Insulin, 0.5ug/mL Hydrocortisone, and 20ng/mL EGF. They looked great, froze...

19 April 2022 7,450 0 View