Sophia Hyman

4 Questions 3 Answers 0 Followers

Questions related from Sophia Hyman

How has human factor VIII cDNA been reverse transcribed?

I have been trying to amplify the human factor VIII mRNA sequence (7Kb) by reverse transcription PCR. I attempted to do this by dividing the sequence into 5 overlapping fragments similar in...

17 October 2020 4,420 2 View

How do I analyse/interpret MUSCLE alignment results?

I performed an alignment of Sanger sequencing results of a piece of DNA. So I aligned the Sanger sense and antisense strand of DNA with a reference sequence. I need to discuss these results in a...

02 October 2020 5,668 4 View

Why is the gel band smaller than expected?

I have been trying to amplify a sequence of 2.2Kb by reverse transcription PCR from the HepG2 cell line. The band size I get is below 2Kb (close to 1.5Kb). I performed touchdown PCR and I added...

30 September 2020 9,606 5 View

How do I design primers that begin outside the coding region of DNA sequence?

I would like to design primers that are complimentary or begin in the UTRs (5' and the 3') of a gene? Where exactly and how do I find the coding sequence that includes the UTRS ?

29 September 2020 4,901 4 View