Does anyone have a better method for generating BM derived pDCs than driving the culture with Flt3L? Or advice to get the cell yield above 10%?

My normal protocol is Flt3L (100ng/ml) supplemented culture media (10% FCS, PSG and 2me in RPMI) driven for 7 days (half the media replenished at day 3 or 4). Any reference to a better method or...

06 June 2015 8,611 2 View