Sooyong Park

6 Questions 5 Answers 0 Followers

Questions related from Sooyong Park

5xFAD Non-Tg mouse behavior is worse than Tg mouse behavior?

Test group -5xFAD female 4 month-age Tg (n=8) -5xFAD female 4 month-age Non-Tg (littermate of Tg) (n=7) (Amyloid beta is only generated in Tg mouse, not Non-Tg) Behavior test -Morris Water maze...

04 June 2019 9,283 4 View

Are there any methods to block non- specific secondary antibody binding in western blot?

•Samples (1, 2, 3, 4 in the figure) are mouse brain tissues. •Primary Ab is mouse IgG. •Secondary Ab is anti-mouse IgG-HRP. •Correct brand size (91kb) is detected, but there are non-specific...

30 January 2019 2,734 2 View

Can I use Alzheimer's disease mouse 5XFAD carrying Pde6brd1 mutation in morris water maze test?

While breeding 5xFAD, there is Pde6brd1 mutant homozyous which cause retinal degeneration . That mutation degenerates eye rod cells which detect bright and dark. Can I use that mouse in morris...

26 July 2018 8,392 0 View

Can I dilute Taq polymerasea and freeze it?

Hello, I use Taq polymerase, Recombinant (Invitrogen, #10342020) In the its protocol, 0.1ul Taq is needed for 1 rxn of 25ul. But it is hard to take 0.1ul. So I think that I dilute Taq in 10 times...

04 December 2017 5,002 3 View

When is appropriate for compound Intraperitoneal injection after Intraperitoneal injection of anesthetic?

I am going to inject compound (rose bengal) intraperitoneally after anesthesia. I will inject avertin intraperitoneally for anesthesia. I am afraid of that the two chemicals; rose bengal and...

27 December 2016 7,811 2 View

What could possibly be the reason of mouse death after phototrombotic ischemia?

Hello, I am trying to make phototrombotic ischemic stroke mouse model. The procedure is 1. Intraperitoneal injection of Rose bengal 50ug/mouse weight (g) 2. After 5 min, switch on the cold light...

22 December 2016 8,539 4 View