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Questions related from Soon Bin Kwon
I found there wasn't termination for antibiotic resistant gene transcription in many vectors. I think stopless transcription will affect activation of following replication origin or another...
18 January 2017 1,993 4 View
I'm planning to separate proteins by size in total lysate(E. coli) by Amicon Ultra 0.5 - 30K, 50K, 100K (Millipore, UFC503008UFC505008UFC510008). I heard total lysate would impair gel filtration...
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Does any one know copy number of pCDF, pRSF, pSC101 in E. coli? And i also want to know compatibility with pUC19 and p15A ori in E. coli. The purpose is using 3 different plasmid, and i have...
30 September 2016 1,348 5 View