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I found there wasn't termination for antibiotic resistant gene transcription in many vectors. I think stopless transcription will affect activation of following replication origin or another...
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Does any one know copy number of pCDF, pRSF, pSC101 in E. coli? And i also want to know compatibility with pUC19 and p15A ori in E. coli. The purpose is using 3 different plasmid, and i have...
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