Sonja Lindfors

5 Questions 17 Answers 0 Followers

Questions related from Sonja Lindfors

AMPK phosphorylation increased in response to high glucose - what's wrong?

I have been culturing human podocytes in normal media (RPMI+10%FBS+ITS+L-glut) and high glucose media (same, but final glucose concentration of 30 mM). Every time I have seen that high glucose...

09 April 2014 7,282 14 View

How can I optimize transfer efficiency from 15 % gel for small and medium-size proteins?

Is there any way to do efficient transfer from 15% SDS PAGE gel for proteins of 14/16 kDa in size AND 62 kDa in size. I'm using wet tank transfer method and Immobilon-FL membrane (PVDF). My...

31 March 2014 6,929 5 View

How can I best carry out a long-lived protein degradation assay?

Hi, I'm planning to perform a long-lived protein degradation assay on my cells. I have found several protocols of which the basic idea is the same but there are some differences. The biggest...

20 March 2014 9,337 5 View

3-MA has no effect on LC-II levels?

I tested 3-MA on human podocytes for the first time but couldn't see any downregulation in LC-II levels. Actually, there was a minor increase in band intensities of 3-MA-treated cells. I tried 2h...

03 January 2014 899 22 View

Any suggestions for the interpretation of three bands between ~12-18 kDa detected with LC3B antibody?

I'm studying autophagy in human podocytes (glomerular epithelial cells) and using rabbit polyclonal LC3B antibody from Sigma. I have been studying the optimal rapamycin concentration to induce...

10 December 2013 3,485 8 View