Sonja Andersen

2 Questions 2 Answers 0 Followers

Questions related from Sonja Andersen

Seahorse assay: when is the OCR too high so the O2 level (mmHg) gets too low (unintended hypoxia during the run)?

During Seahorse mitostress assays, we have great variance between different cell lines regarding their oxygen consumption. One cell line is tricky because at relevant confluency, the basal OCR is...

24 August 2021 2,991 2 View

CyQuant cell prolif. assay - how old can it be and still work??

Hi, I have frozen two 96-well plates with cells that I need to stain using the CyQuant Cell Proliferation Assay from ThermoFisher (C7026). However, I didn't realize that we only have some very old...

07 January 2021 384 4 View