Sonia Shastri

4 Questions 5 Answers 0 Followers

Questions related from Sonia Shastri

Does anybody has good protocol for Lipid Peroxidation assay?

I want to measure lipid peroxdation level in mouse colon tissue homogenate. Can anyone help me out in this?

03 March 2017 9,426 11 View

Is knocking down of NLRP3 affects the ROS level in lamina propria macrophages in mouse model of cancer?

I want to test antioxidant drug in spontaneous colorectal cancer mouse model. By knocking down NLRP3 inflammasome, mice which already having spontaneous colitis develops tumor in proximal colon...

25 October 2016 701 2 View

How i do combination studies of wnt inhibitors with chemo drugs by MTT assay ?

I want to do combination studies of various wnt inhibitors with chemotherapeutic drugs. I have calculated their IC50 values by MTT assay. Now i am confused how to design combinations. I am...

29 July 2015 8,769 7 View

Is there any effect of incubating colorectal cells lines for long hours with MTT solution ?

According to my protocol i have to incubate my plate for 1 hr after adding MTT solution. but i incubated for 2.45 hours. My results were not good. Some variations in the OD values. My cell density...

26 May 2015 7,180 3 View