Sonali Bhadra

2 Questions 7 Answers 0 Followers

Questions related from Sonali Bhadra

Having trouble in western blotting of 15KDa histone. Getting blow through.Can anybody suggest a solution?

Hi I am having trouble in western blotting of 15 KDa histone. I am using 12% gel for SDS PAGE where my bands resolve very sharpely. Earlier I had used 0.45µM Nitrocellulose membrane for 1hr at...

10 October 2017 9,687 6 View

How can I troubleshoot P1 phage transduction?

I am trying to carry out gene deletion using P1 phage transduction in Ecoli W3110 Strain. Here I have successfully generated lysate from the donor strain with pfu 1.0E+9/ml via plate method. I am...

02 February 2015 2,280 8 View