How does decreasing the height of sucrose cushion (1M, no gradient) effect ultracentrifugation / recovery?

I am aim to pellet ribosome footprints (ribosome bound to ~30nt mRNA fragment) by layering the cell lysate on 0.8ml of 1M sucrose cushion (no gradient) through ultracentrifugation at 71,000rpm for...

19 March 2018 9,135 0 View