Sonal Jaiswal

3 Questions 2 Answers 0 Followers

Questions related from Sonal Jaiswal

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

19 February 2021 6,955 4 View

Cloning a 4.3kb insert into 6kb vector?

I really need tips for cloning 5kb inserts into 6kb vector. I am trying the cut n paste method using REs. Which ligation ratio should I use? What precautions should I consider? I would be really...

25 November 2019 9,545 2 View

Inserting Gene of interest in vector with internal restriction sites?

I am trying to clone two fragments into my vector of interest pcdna5 frt to. I have insertedone fragment into the vector using cut and paste method. Now i want to insert another fragment into the...

18 May 2019 8,635 0 View