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I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...
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I really need tips for cloning 5kb inserts into 6kb vector. I am trying the cut n paste method using REs. Which ligation ratio should I use? What precautions should I consider? I would be really...
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