Does anyone have any idea about how to enhance the efficiency of TEV protease cleavage of a fusion protein ?

Hi All, I have a fusion protein (fused with a N-term His6 Tag) that I want to try the TEV cleavage on (ENLYFQ/C), cleavage is expected to happen between the Q and C. I tried different conditions...

02 November 2017 7,657 19 View