I have a 5ml culture bacterial pellet, that I stored in -20 over night to work later. I only need 40ul. But the pellet wont resuspend. Help?

I am trying to purify a protein that is MBP tagged. This protein, is known in literature to be insoluble. I have employed the pMAL-C2x vectore for the same. It has a lac inducible promoter. Now, I...

06 April 2018 5,889 3 View