Soham Gupta

2 Questions 4 Answers 0 Followers

Questions related from Soham Gupta

Can I run a native gel from frozen protein lysates?

I am looking for protein complexes and was wondering whether I can run a native gel followed by western from previously frozen protein lysates which are in -20 for 2 weeks.

30 January 2017 4,948 4 View

Can DMEM be used rather than MEM 2x while mixing with agarose during a plaque assay?

I am referring to current protocols in Microbiology 15D.3.1-15D.3.4 for plaque assay of West Nile Virus. As per the protocol the agarose should be diluted at 1:1 ratio with 2X MEM+8%FBS. However I...

23 June 2014 867 8 View