Sofia Omari

2 Questions 8 Answers 0 Followers

Questions related from Sofia Omari

Is there a way to preserve PBMCs for later protein extraction and western blotting?

I would like to collect PBMCs from patients to later extract protein and do western blotting eventually. I wonder if there is a way to preserve the protein or the cells (in the field), so my...

03 March 2013 5,162 7 View

Is it possible to do flow cytometry on frozen/thawed PBMCs?

I wonder if I can thaw a frozen PBMCs from nitrogen phase, wash them and surface or fix/perm and internally immunostain them and test them with flow cytometry? I tried to do surface staining on...

03 March 2013 1,673 2 View