Sofia Visconti

4 Questions 6 Answers 0 Followers

Questions related from Sofia Visconti

Percentage of agarose gel to discriminate 700 from 800bp digestion product?

Hello, do you have any suggestion on what is the optimal agarose percentage to discriminate between a digestion product of 700 from 800 bp? Is there any other tip (like using a slightly higher or...

11 October 2023 927 5 View

Capillary electrophoresis Quiaxcel advanced: does the buffer matter?

Hello, I am trying to use the Quiaxcel advanced capillary electrophoresis system for fragment analysis of PCR products that have been cut with a restriction enzyme. I dissolve my ladder (size...

05 April 2023 5,047 5 View

Sanger sequencing of PCR product - How to improve quality?

Hello, I am conducting CRISPR experiments on some cell lines. At the end of the experiment, I harvest the DNA, amplify with PCR the region of interest and I send it to a company that does Sanger...

18 February 2022 2,475 9 View

Growth factor frozen in PBS, does the protein degrade?

I froze (-20C) an aliquot of growth factor (FGF7) in PBS instead of BSA 0.1%. Do you think I should bin it or would it still work?

16 November 2021 6,732 4 View