Can anyone help with a ds cDNA synthesis cloning problem?

Hi, I have been using Thermo Scientifics Maxima H Minus cDNA double stranded synthesis kit to generate dscDNA.  I carried out 2 rxns with oDT and RH primers (10 pmol) and got ~10 kb band for oDT...

09 July 2014 6,384 14 View