Snigdha Guha

2 Questions 2 Answers 0 Followers

Questions related from Snigdha Guha

How can I induce the expression of inflammatory biomarkers in HUVECs using the commercial ox-LDL?

I am doing an in-vitro atherosclerotic study in my lab using HUVECs. I am using a commercial ox-LDL from Thermo Fischer (L34357) to induce the expression of inflammatory biomarkers such as ICAM-1,...

28 July 2021 4,140 3 View

Induction of inflammation using TNF-alpha in HUVEC cells isn't happening. Any suggestions on what changes to imply?

I have been trying to induce inflammation in HUVEC cells using TNF-alpha (5ng/mL) for 4 hrs. However, as the passage number increases the induction is not so strong. I check using ICAM-1 and...

26 July 2018 7,425 2 View