Smriti Chand

4 Questions 8 Answers 0 Followers

Questions related from Smriti Chand

Fluo4AM staining observed in DMSO negative control. Any suggestions to reduce the background?

I'm new to the Calcium measurements using the Fluo4AM dye. I'm working on titrating the amount of Fluo-4AM dye for measuring the Calcium release from HEK-293T cells. I used Thapsigargin as...

13 August 2024 5,450 1 View

Suggestions for specific markers for cytoplasmic, nuclear and membrane fractions for western blot analysis?

I did the subcellular fractionation of transfected HEK-293T cells. For western blot analysis for the purity of fractions, I used anti-B actin antibody, anti-fibrillarin G4 antibody and...

19 July 2023 1,292 2 View

What buffers to be used for purification of his-tagged protein from Expi-CHO cell pellet?

We were working on purification of a his-tagged protein from Expi-CHO cell pellet and tried different buffers along with sonication to get the protein out in the supernatant for further...

19 May 2023 7,691 5 View

Can we use TEV protease to cleave the His-tag from fusion protein in a transfected cell lysate sample?

I have came across the protocols that define the cleavage of His-tag from His-tagged fusion proteins with TEV protease using purified protein samples. I was wondering if we can apply the protocol...

09 November 2022 8,800 4 View