Smriti Chand

3 Questions 6 Answers 0 Followers

Questions related from Smriti Chand

Suggestions for specific markers for cytoplasmic, nuclear and membrane fractions for western blot analysis?

I did the subcellular fractionation of transfected HEK-293T cells. For western blot analysis for the purity of fractions, I used anti-B actin antibody, anti-fibrillarin G4 antibody and...

19 July 2023 1,266 2 View

What buffers to be used for purification of his-tagged protein from Expi-CHO cell pellet?

We were working on purification of a his-tagged protein from Expi-CHO cell pellet and tried different buffers along with sonication to get the protein out in the supernatant for further...

19 May 2023 7,671 5 View

Can we use TEV protease to cleave the His-tag from fusion protein in a transfected cell lysate sample?

I have came across the protocols that define the cleavage of His-tag from His-tagged fusion proteins with TEV protease using purified protein samples. I was wondering if we can apply the protocol...

09 November 2022 8,780 4 View