Why I am getting two bands on agarose gel after pcr amplification of a mini prep plasmid having gene of interest with gene specific primers?

I have a purified plasmid having the insert of 655 bp. when i am pcr amplifying it with gene specific primer ideally i should get single amplified band of size 655 bp but after running it on gel i...

27 July 2019 1,950 7 View