Smita Kujur

5 Questions 6 Answers 0 Followers

Questions related from Smita Kujur

Has anyone used the TAKARA gel electrophoresis set? I wanted of it's good and what's your reviews about that instrument?

Any reviewers for that instrument. Please share your feedback. I was thinking of ordering but I'm doubtful.

08 March 2019 8,782 0 View

No amplification after PCR?

I successfully amplified my samples using Primer set 1 but when I'm amplifying the same samples using different Primer set, the genomic DNA is still stuck in the Wells and only ladder resolves. My...

10 July 2018 9,383 13 View

Is there a substitute for Potassium Bicarbonate?

I was using Potassium Bicarbonate to make my RBC lysis buffer but now that salt is no more available in market. Is there a substitute for it? It was made along with Ammonium Chloride and Disodium...

28 June 2017 958 3 View

Why do we use 3% Igepal in FISH cytogenetics?

We use wash solution on the 2nd day of FISH experiment. It contains SSC + 3% Igepal , a non-denaturing detergent. Can you specify what is it's action on the slides?

22 August 2016 4,505 5 View

Protocol for isolating DNA from the pellet obtained after the harvested cells from cell culture done by cytogenetic method?

Can somebody give me a proper protocol for isolating DNA from the pellet obtained after the harvested cells from cell culture done by cytogenetic method. The pellet is in 3:1 fixative (Clarke's...

02 August 2016 9,114 4 View