Smarth Narula

2 Questions 1 Answers 0 Followers

Questions related from Smarth Narula

Why is the addition of Insulin killing my HEK293 cells?

I am currently culturing some HEK cells with absolutely no issue, they are being cultured with the standard DMEM + 10% FBS media. For a particular experiment, I need to introduce exogenous...

22 July 2021 4,204 3 View

Is electroporation the only viable way to produce a knockout in hESCs?

If anyone could help, it would be greatly appreciated. I am currently in the middle of introducing a targeted knockout in human embryonic stem cells - I am using the plasmid px459v2 which has has...

23 March 2021 3,777 2 View