Smaranda Badea

2 Questions 5 Answers 0 Followers

Questions related from Smaranda Badea

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

16 February 2021 3,035 2 View

Can anyone advise on OPC culture issues - cell debris and low attachment?

I used to get very clean rat OPC cultures via neurospheres, but for the past year or so (multiple batches overall) I've noticed lower viability in the NPC to OPC phase and I can't figure out where...

26 February 2020 559 0 View